pTSlb-R756S

60730 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
Depositor
Matthew Bennett
Article
Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
Mutation
Residues 1-180 of split T7 RNAP and Residues 180-…
Available Since
Jan. 16, 2015
Availability
Available

Sequence Information

Length
829 bp
Topology
linear
Molecule Type
genomic DNA

Features

No features annotated for this plasmid.