pTSara-N748D

60726 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
Depositor
Matthew Bennett
Article
Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
Mutation
Residues 1-180 of split T7 RNAP and Residues 180-…
Available Since
Oct. 2, 2015
Availability
Available

Sequence Information

Length
721 bp
Topology
linear
Molecule Type
genomic DNA

Features

terminator 1
Label Gene Product Position Strand
T7Te terminator 340..368 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG