M26L DJ-1 TEV site pET15b

60686 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Recombinant protein expression of Park7
Depositor
Mark Wilson
Article
Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do
Insert
M26L DJ-1 (PARK7 Human)
Tags
6xHis
Mutation
M26L and TEV site was inserted (see comments belo…
Promoter
T7
Available Since
Oct. 24, 2014
Availability
Available

Sequence Information

Length
738 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 588..609 Rev
DNA Sequence
GAAAACCTGTATTTTCAGTCT
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 642..660 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
protein_bind 1
Label Gene Product Position Strand
lac operator — — 719..736 Fwd
DNA Sequence
TTGTTATCCGCTCACAA