M26I DJ-1 TEV site pET15b

60685 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Recombinant protein expression of Park7
Depositor
Mark Wilson
Article
Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do
Insert
M26I DJ-1 (PARK7 Human)
Tags
6xHis
Mutation
M26I and TEV site was inserted (see comments belo…
Promoter
T7
Available Since
Oct. 24, 2014
Availability
Available

Sequence Information

Length
616 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 28..46 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 55..73 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
TEV site tobacco etch virus (TEV) protease recog… 79..100 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGTCT
Protein Sequence
ENLYFQS