M26I DJ-1 pET15b

60677 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Recombinant protein expression of Park7
Depositor
Mark Wilson
Article
Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.
Insert
M26I DJ-1 (PARK7 Human)
Tags
6xHis
Mutation
M26I
Promoter
T7
Available Since
Oct. 24, 2014
Availability
Available

Sequence Information

Length
714 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 41..59 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 68..86 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS