pCT

60620 Streptococcus pyogenes Yeast Expression

Plasmid Information

Purpose
Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
Depositor
Huimin Zhao
Article
Bao et al ACS Synth Biol. 2014 Sep 19.
Tags
FLAG and SV40 NLS
Mutation
changed Aspartate 147 to Tyrosine, Proline 411 to…
Promoter
RPR1p and TEF1p
Available Since
Nov. 7, 2014
Availability
Available

Sequence Information

Length
583 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
FLAG — FLAG(R) epitope tag, followed by an ent… 27..51 Fwd
DNA Sequence
GATTATAAAGATGACGATGACAAA
Protein Sequence
DYKDDDDK
SV40 NLS — nuclear localization signal of SV40 (si… 54..75 Fwd
DNA Sequence
CCAAAAAAGAAGAGAAAGGTC
Protein Sequence
PKKKRKV