myc-actin-NES-1

60618 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes MYC tagged beta-actin with a mutation in the NES
Depositor
Primal de Lanerolle
Article
Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.
Insert
Beta-actin (ACTB Human)
Tags
MYC
Mutation
I178A, L180A
Available Since
Nov. 21, 2014
Availability
Available

Sequence Information

Length
877 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 50..80 Fwd
DNA Sequence
GAACAAAAACTCATCTCAGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
T7 promoter — — 5..24 Fwd
DNA Sequence
TAATACGACTCACTATAGG