pCas_5+7_gfp

60595 Synthetic Bacterial Expression

Plasmid Information

Purpose
encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.
Depositor
Christopher Voigt
Article
Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi
Insert
Cas_5+7_gfp
Available Since
April 28, 2015
Availability
Available

Sequence Information

Length
836 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
J23119 promoter — — 120..155 Rev
DNA Sequence
TTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGC
terminator 2
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 272..344 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator — — 359..387 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG