UbcH5B — PlasmidDB

Plasmid Information

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Promoter

Available Since

Jan. 29, 2015

Availability

Available

Length

722 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	141..159	Fwd
DNA Sequence CATCATCATCATCATCAT Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	47..66	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	66..91	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC