pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

60405 Synthetic Yeast Expression

Plasmid Information

Purpose
Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
Depositor
Ron Vale
Article
Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
Insert
TUB2
Mutation
TUB2 amino acids 429 - end, replaced with human T…
Promoter
GAL
Available Since
Jan. 26, 2015
Availability
Available

Sequence Information

Length
827 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 665..674 Fwd
DNA Sequence
ACCTCCTTA
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 19..268 Rev
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAAACCTGGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC