pRS424-TUB2 - human TUBB6 Cterminal tail

60403 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter
Depositor
Ron Vale
Article
Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
Insert
TUB2
Mutation
TUB2 amino acids 429 - end, replaced with human T…
Promoter
GAL
Available Since
May 6, 2015
Availability
Available

Sequence Information

Length
768 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 662..671 Fwd
DNA Sequence
ACCTCCTTA
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 11..259 Rev
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC