pRS424-TUB2 - human TUBB1 Cterminal tail

60400 Synthetic Yeast Expression

Plasmid Information

Purpose
Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter
Depositor
Ron Vale
Article
Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
Insert
TUB2
Mutation
TUB2 amino acids 429 - end, replaced with human T…
Promoter
GAL
Available Since
May 6, 2015
Availability
Available

Sequence Information

Length
798 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
GAL1 promoter S. cerevisiae GAL1 — 15..457 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG
protein_bind 1
Label Gene Product Position Strand
UAS — — 15..133 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG