pRS424-TUB2 (1-428)

Plasmid Information

Purpose

Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Mutation

deleted 429 - end

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Available

Length

809 bp

Topology

linear

Molecule Type

genomic DNA

RBS 1

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	725..734	Fwd
DNA Sequence ACCTCCTTA

terminator 1

Label	Gene	Product	Position	Strand
CYC1 terminator	S. cerevisiae CYC1	—	17..265	Rev
DNA Sequence TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC