pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

60394 Synthetic Yeast Expression

Plasmid Information

Purpose
Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
Depositor
Ron Vale
Article
Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
Insert
TUB1 (TUB1 Budding Yeast, Synthetic)
Mutation
internal 6xHis (see supplement figure 4), amino a…
Promoter
GAL
Available Since
Jan. 26, 2015
Availability
Available

Sequence Information

Length
765 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 11..259 Rev
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC