pRS424-TUB2

60388 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Expression of yeast beta tubulin (TUB2) using a GAL promoter
Depositor
Ron Vale
Article
Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
Insert
TUB2
Promoter
GAL
Available Since
Jan. 26, 2015
Availability
Available

Sequence Information

Length
807 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
GAL1 promoter S. cerevisiae GAL1 — 24..466 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG
protein_bind 1
Label Gene Product Position Strand
UAS — — 24..142 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG