Gal4pBS12-Pleum — PlasmidDB

Plasmid Information

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Available Since

Feb. 11, 2015

Availability

Available

Length

740 bp

Topology

linear

Molecule Type

genomic DNA

promoter 2

Label	Gene	Product	Position	Strand
lac promoter	—	—	359..390	Fwd
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG
T3 promoter	—	—	458..477	Fwd
DNA Sequence AATTAACCCTCACTAAAGG

protein_bind 2

Label	Gene	Product	Position	Strand
CAP binding site	—	—	323..345	Fwd
DNA Sequence TAATGTGAGTTAGCTCACTCAT
lac operator	—	—	397..414	Fwd
DNA Sequence TTGTGAGCGGATAACAA