pNB689

60133 Synthetic Yeast Expression

Plasmid Information

Purpose
Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
Depositor
Nicolas Buchler
Article
Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
Insert
GrLuc
Tags
ClpXP degron and Nuclear localization signal (NLS…
Promoter
MET17pr
Available Since
Oct. 31, 2014
Availability
Available

Sequence Information

Length
826 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
SV40 NLS — nuclear localization signal of SV40 (si… 486..507 Fwd
DNA Sequence
CCAAAAAAAAAAAGAAAAGTT
Protein Sequence
PKKKRKV
promoter 2
Label Gene Product Position Strand
T3 promoter — — 24..43 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
MET17 promoter S. cerevisiae MET17 — 93..443 Fwd
DNA Sequence
TTATTTTTTGCTTTTTCTCTTGAGGTCACATGATCGCAAAATGGCAAATGGCACGTGAAGCTGTCGATATTGGGGAACTGTGGTGGTTGGCAAATGACTAATTAAGTTAGTCAAGGCGCCATCCTCATGAAAACTGTGTAACATAATAACCGAAGTGTCGAAAAGGTGGCACCTTGTCCAATTGAACACGCTCGATGAAAAAAATAAGATATATATAAGGTTAAGTAAAGCGTCTGTTAGAAAGGAAGTTTTTCCTTTTTCTTGCTCTCTTGTCTTTTCATCTACTATTTCCTTCGTGTAATACAGGGTCGTCAGATACATAGATACAATTCTATTACCCCCATCCATAC