pTHSSd_41 — PlasmidDB

Plasmid Information

Purpose

High full length T7 RNAP* expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7* RNAP

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 19, 2014

Availability

Available

Length

383 bp

Topology

linear

Molecule Type

genomic DNA

misc_feature 1

Label	Gene	Product	Position	Strand
MCS	—	—	125..182	Fwd
DNA Sequence AAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTC

promoter 3

Label	Gene	Product	Position	Strand
lac promoter	—	—	4..35	Fwd
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG
SP6 promoter	—	—	100..119	Fwd
DNA Sequence ATTTAGGTGACACTATAGA
T7 promoter	—	—	184..203	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	42..59	Fwd
DNA Sequence TTGTGAGCGGATAACAA