pET14b_tev_PDZ1

59739 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expresses PDZ1 domain of human ZO-1 in bacteria
Depositor
Alan Fanning
Insert
zonula occludens-1 (TJP1 Human)
Tags
HIS tag and thrombin clvg
Available Since
Sept. 11, 2014
Availability
Available

Sequence Information

Length
698 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 139..157 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 172..193 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGC
Protein Sequence
ENLYFQG
terminator 1
Label Gene Product Position Strand
T7 terminator — — 542..590 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG