pGEX-2T_PDZ2

59735 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expresses PDZ2 domain of human ZO-1 in bacteria
Depositor
Alan Fanning
Article
Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6
Insert
zonula occludens-1 (TJP1 Human)
Tags
GST and thrombin clvg
Available Since
Sept. 18, 2014
Availability
Available

Sequence Information

Length
811 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
ISS — — 394..773 Fwd
DNA Sequence
AACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTT
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 105..210 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT