pET14b_tev_PSG — PlasmidDB

Plasmid Information

Purpose

Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

TJP1 (TJP1 Human)

Length

719 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	146..164	Fwd
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	179..200	Fwd
DNA Sequence GAGAATCTTTATTTTCAGGGC Protein Sequence ENLYFQG

RBS 1

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	655..664	Fwd
DNA Sequence TAAGGAGGT

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	54..73	Fwd
DNA Sequence TAATACGACTCACTATAGG