pET-21a-GFP-Patronin CC 534–868

59054 Drosophila melanogaster Bacterial Expression

Plasmid Information

Purpose
for E. coli expression of Patronin CC truncation
Depositor
Ron Vale
Article
Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11
Insert
Patronin (Patronin Fly)
Tags
GFP
Available Since
Aug. 27, 2014
Availability
Available

Sequence Information

Length
773 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 11..29 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 38..56 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 136..155 Rev
DNA Sequence
TAATACGACTCACTATAGG
lacI promoter lacI — 463..541 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 111..136 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC