pET-28a+GFP-CAMSAP3 CKK

59033 Mus musculus Bacterial Expression

Plasmid Information

Purpose
expresses CKK domain of CAMSAP3 in E. coli
Depositor
Ron Vale
Article
Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11
Insert
CAMSAP3 CKK domain (CAMSAP3 Mouse)
Tags
His, Strep, TEV, and sfGFP
Available Since
Sept. 2, 2014
Availability
Available

Sequence Information

Length
772 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 6..27 Fwd
DNA Sequence
GAAAATCTGTATTTTCAGGGG
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 585..603 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 669..717 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG