pET-28a+GFP-CAMSAP2 CKK

59032 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expresses CKK domain of CAMSAP2 in E. coli
Depositor
Ron Vale
Article
Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11
Insert
CAMSAP2 CKK domain (CAMSAP2 Human)
Tags
His, Strep, TEV, and sfGFP
Available Since
Aug. 27, 2014
Availability
Available

Sequence Information

Length
700 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 466..484 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 550..598 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG