pEVA1

58953 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
Depositor
Thomas Fox, Thomas Mason
Article
Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
Insert
VAR1u, VAR1 recoded for expression from the nucleus
Tags
preCox4 mitochondrial matrix targeting signal
Mutation
Synthetic gene in standard genetic code
Promoter
ADH1
Available Since
Aug. 15, 2014
Availability
Available

Sequence Information

Length
636 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
pre-CoxIV — mitochondrial presequence of yeast cyto… 155..221 Fwd
DNA Sequence
ATGCTTTCACTACGTCAATCTATAAGATTTTTCAAGCCAGCCACAAGAACTTTGTGTAGCTCTAGA
Protein Sequence
MLSLRQSIRFFKPATRTLCSSR