pRS406-PHO5-GFP-OSBP PH domain

Plasmid Information

Purpose

Expresses GFP-Tagged OSBP PH domain in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Length

748 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
Myc	—	Myc (human c-Myc proto-oncogene) epitop…	13..43	Fwd
DNA Sequence GAGCAAAAGCTCATTTCTGAAGAGGACTTG Protein Sequence EQKLISEEDL

promoter 2

Label	Gene	Product	Position	Strand
T3 promoter	—	—	387..406	Rev
DNA Sequence AATTAACCCTCACTAAAGG
lac promoter	—	—	474..505	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	450..467	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	519..541	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA