pRS406-PHO5-GFP-CERT PH domain EE mutation

58833 Homo sapiens Yeast Expression

Plasmid Information

Purpose
Expresses GFP-Tagged and mutated CERT PH domain in yeast
Depositor
Tim Levine
Article
Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9
Insert
collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
Tags
GFP and Myc
Mutation
changed Arginine 43 to Glutamate
Promoter
PHO5
Available Since
Aug. 27, 2014
Availability
Available

Sequence Information

Length
872 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 430..460 Rev
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
T3 promoter — — 7..26 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG