pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

58830 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Expresses GFP-Tagged and mutated Osh2 PH domain in yeast
Depositor
Tim Levine
Article
Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9
Insert
2 x Osh2 PH domain (OSH2 Budding Yeast)
Tags
GFP and Myc
Mutation
changed Lysine 307 to Glutamate and Arginine 309 …
Promoter
PHO5
Available Since
Sept. 30, 2014
Availability
Available

Sequence Information

Length
799 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 1
Label Gene Product Position Strand
T3 promoter 7..26 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG