pRS406-PHO5-GFP-2xOsh2 PH domain [short]

58829 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Expresses GFP-Tagged Osh2 PH domain in yeast
Depositor
Tim Levine
Article
Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9
Insert
2xOsh2 PH domain [short] (OSH2 Budding Yeast)
Tags
GFP and Myc
Promoter
PHO5
Available Since
Aug. 27, 2014
Availability
Available

Sequence Information

Length
826 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 733..763 Rev
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
T3 promoter — — 10..29 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG