SDC229-B03

58763 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PDB 4PYZ
Depositor
Cheryl Arrowsmith
Insert
PDB:4PYZ (USP7 Human)
Tags
His
Mutation
Bacterial expression for structure determination;…
Promoter
T7
Available Since
Aug. 5, 2014
Availability
Available

Sequence Information

Length
885 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 29..47 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 56..74 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS