pRS406-PHO5-GFP-CERT PH domain

58725 Homo sapiens Yeast Expression

Plasmid Information

Purpose
Expresses GFP-Tagged CERT PH domain in yeast
Depositor
Tim Levine
Article
Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.
Insert
collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
Tags
GFP and Myc
Promoter
PHO5
Available Since
Aug. 27, 2014
Availability
Available

Sequence Information

Length
815 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 24..54 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
promoter 2
Label Gene Product Position Strand
T3 promoter — — 458..477 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 545..576 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 521..538 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 590..612 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA