pET-20b-M-Ntx-S1

58716 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S
Depositor
Fiorella Tonello
Article
Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14
Insert
Notexin
Tags
Methionine
Mutation
Changed Asparagine 1 to Serine
Promoter
T7
Available Since
Aug. 11, 2014
Availability
Available

Sequence Information

Length
754 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 431..449 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 515..563 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG