pET-20b-T7-Ntx-K49

58715 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K
Depositor
Fiorella Tonello
Article
Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14
Insert
Notexin
Tags
T7 peptide tag
Mutation
Changed Aspartic Acid 49 to Lysine
Promoter
T7
Available Since
Aug. 11, 2014
Availability
Available

Sequence Information

Length
819 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
Factor Xa site Factor Xa recognition and cleavage site 55..67 Fwd
DNA Sequence
ATCGAAGGCCGC
Protein Sequence
IEGR
6xHis 6xHis affinity tag 473..491 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator 557..605 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG