pET28a-ybbR-HIS-Xyl-DocI

58711 Synthetic Bacterial Expression

Plasmid Information

Purpose
Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
Depositor
Hermann Gaub
Article
Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
Insert
Xyl-DocI
Tags
HIS, HRV 3C, and ybbR
Mutation
changed Threonin 120 to Cysteine in Xylanase Doma…
Promoter
T7
Available Since
Aug. 25, 2014
Availability
Available

Sequence Information

Length
855 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 181..205 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGTCCG
Protein Sequence
LEVLFQGP
6xHis — 6xHis affinity tag 211..229 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 51..70 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 70..95 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC