pET28a-StrepII-TagRFP-CohI

58710 Synthetic Bacterial Expression

Plasmid Information

Purpose
Detection construct to label dockerin type I containing fusion proteins
Depositor
Hermann Gaub
Article
Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
Insert
TagRFP-CohI
Tags
StrepII
Promoter
T7
Available Since
Aug. 25, 2014
Availability
Available

Sequence Information

Length
779 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
Strep-Tag II — peptide that binds Strep-Tactin(R), an … 137..161 Fwd
DNA Sequence
TGGTCTCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEK
promoter 1
Label Gene Product Position Strand
T7 promoter — — 46..65 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 65..90 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC