pTRC-APX(W41F) dimeric

58499 Synthetic Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of APX W41F inducible by IPTG
Depositor
Alice Ting
Article
Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11
Insert
pea APX W41F (dimeric)
Tags
His6
Mutation
W41F
Promoter
trc
Available Since
Aug. 5, 2014
Availability
Available

Sequence Information

Length
1,115 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 650..668 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
trc promoter — — 570..600 Fwd
DNA Sequence
TTGACAATTAATCATCCGGCTCGTATAATG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 607..624 Fwd
DNA Sequence
TTGTGAGCGGATAACAA