pSEVA12TEC-B

58307 Synthetic Bacterial Expression

Plasmid Information

Purpose
GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.
Depositor
Tom Ellis
Article
Wright et al ACS Synth Biol. 2014 May 13.
Insert
Genomic cassette
Available Since
March 6, 2015
Availability
Available

Sequence Information

Length
860 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

RBS 2
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 714..723 Fwd
DNA Sequence
ACCTCCTTA
Shine-Dalgarno sequence — — 763..772 Fwd
DNA Sequence
ACCTCCTTA
protein_bind 1
Label Gene Product Position Strand
FRT (minimal) — — 386..420 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC