pSEVA12DKR-J

58306 Synthetic Bacterial Expression

Plasmid Information

Purpose
GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.
Depositor
Tom Ellis
Article
Wright et al ACS Synth Biol. 2014 May 13.
Insert
Genomic cassette
Available Since
March 6, 2015
Availability
Available

Sequence Information

Length
810 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

RBS 2
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 699..708 Fwd
DNA Sequence
ACCTCCTTA
Shine-Dalgarno sequence — — 747..756 Fwd
DNA Sequence
ACCTCCTTA
protein_bind 1
Label Gene Product Position Strand
FRT (minimal) — — 386..420 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC