pSEVA12DKR-E — PlasmidDB

Plasmid Information

Purpose

GeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Available Since

Dec. 22, 2014

Availability

Available

Length

837 bp

Topology

linear

Molecule Type

genomic DNA

RBS 2

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	692..701	Fwd
DNA Sequence ACCTCCTTA
Shine-Dalgarno sequence	—	—	740..749	Fwd
DNA Sequence ACCTCCTTA

protein_bind 1

Label	Gene	Product	Position	Strand
FRT (minimal)	—	—	379..413	Rev
DNA Sequence GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC