pSEVA12DKR-A — PlasmidDB

Plasmid Information

Purpose

GeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Available Since

Nov. 4, 2014

Availability

Available

Length

925 bp

Topology

linear

Molecule Type

genomic DNA

RBS 2

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	694..703	Fwd
DNA Sequence ACCTCCTTA
Shine-Dalgarno sequence	—	—	742..751	Fwd
DNA Sequence ACCTCCTTA

protein_bind 1

Label	Gene	Product	Position	Strand
FRT (minimal)	—	—	381..415	Rev
DNA Sequence GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC

terminator 1

Label	Gene	Product	Position	Strand
T7Te terminator	—	—	819..847	Rev
DNA Sequence GGCTCACCTTCGGGTGGGCCTTTCTGCG