pET14b-PITPalpha K61A

58258 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of human PITP alpha K61A
Depositor
Shamshad Cockcroft
Article
Tilley et al Structure. 2004 Feb;12(2):317-26.
Insert
PITP alpha (PITPNA Human)
Tags
HIS
Mutation
K61A (abolishes PI binding)
Promoter
T7
Available Since
July 15, 2014
Availability
Available

Sequence Information

Length
575 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 26..44 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 53..71 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS