pET14b-PITPalpha N90F

58257 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of human PITP alpha N90F
Depositor
Shamshad Cockcroft
Article
Tilley et al Structure. 2004 Feb;12(2):317-26.
Insert
PITP alpha (PITPNA Human)
Tags
HIS
Mutation
N90F (abolishes PI binding)
Promoter
T7
Available Since
July 28, 2014
Availability
Available

Sequence Information

Length
525 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 10..28 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 37..55 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS