pET28a(+) 6xHis-3xFlag-IFIT5

53561 Homo sapiens Bacterial Expression

Plasmid Information

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Tags

3xFLAG, 6xHIS, and TEV site

Promoter

T7

Available Since

May 29, 2014

Availability

Available

Sequence Information

Length

1,134 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 4

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	163..181	Fwd
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH
thrombin site	—	thrombin recognition and cleavage site	190..208	Fwd
DNA Sequence CTGGTGCCGCGCGGCAGC Protein Sequence LVPRGS
3xFLAG	—	three tandem FLAG(R) epitope tags, foll…	223..289	Fwd
DNA Sequence GACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGATTACAAGGATGACGATGACAAG Protein Sequence DYKDHDGDYKDHDIDYKDDDDK
TEV site	—	tobacco etch virus (TEV) protease recog…	289..310	Fwd
DNA Sequence GAAAACCTGTATTTTCAGGGC Protein Sequence ENLYFQG

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	64..83	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	83..108	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC