pHB4

53462 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.
Depositor
Elizabeth Conibear
Article
Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11
Insert
yeGFP-Snc1-Suc2
Promoter
ADH
Available Since
May 19, 2016
Availability
Available

Sequence Information

Length
1,134 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 3
Label Gene Product Position Strand
lac promoter 123..154 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
T3 promoter 222..241 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
TEF promoter 542..886 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
protein_bind 2
Label Gene Product Position Strand
CAP binding site 87..109 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 161..178 Fwd
DNA Sequence
TTGTGAGCGGATAACAA