pCB116

53365 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
To express Cup9p‐FLAG from the PGAL1 promoter
Depositor
Alexander Varshavsky
Article
Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.
Insert
Cup9 (CUP9 Budding Yeast)
Tags
Flag
Promoter
GAL1
Available Since
June 3, 2014
Availability
Available

Sequence Information

Length
919 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
T3 promoter — — 7..26 Fwd
DNA Sequence
AATTAACCCTCACTAAAGG
GAL1 promoter S. cerevisiae GAL1 — 49..491 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG
protein_bind 1
Label Gene Product Position Strand
UAS — — 49..167 Fwd
DNA Sequence
CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCG