pZS*11-yfp0

53241 Synthetic Bacterial Expression

Plasmid Information

Purpose
express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)
Depositor
Philippe Cluzel
Article
Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110
Insert
YFP
Promoter
PLtetO1
Available Since
May 4, 2015
Availability
Available

Sequence Information

Length
610 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
PLtetO-1 promoter — — 18..92 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACATCAGCAGGACGCACTGACC
protein_bind 2
Label Gene Product Position Strand
tet operator tetO — 18..37 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA
tet operator tetO — 43..62 Fwd
DNA Sequence
TCCCTATCAGTGATAGAGA