PCRT7 tetR pLtetO MBP-MEK1 XX Amp

53225 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
Depositor
Jesse Rinehart
Insert
MEK1 (MAP2K1 Human)
Tags
HIS and MBP
Mutation
Codon optimized for E.coli, both codons for Ser21…
Promoter
pLtetO
Available Since
May 7, 2014
Availability
Available

Sequence Information

Length
853 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
Factor Xa site — Factor Xa recognition and cleavage site 20..32 Fwd
DNA Sequence
ATCGAGGGAAGG
Protein Sequence
IEGR
TEV site — tobacco etch virus (TEV) protease recog… 32..53 Fwd
DNA Sequence
GAAAATCTGTATTTTCAAGGT
Protein Sequence
ENLYFQG