pGEX4T3-HA-ERK2 GOF

53200 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector
Depositor
Christopher Counter
Article
Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1
Insert
ERK2 (MAPK1 Human)
Tags
GST and HA
Mutation
R67S/D321N
Available Since
June 24, 2014
Availability
Available

Sequence Information

Length
1,057 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 19..37 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
HA HA (human influenza hemagglutinin) epit… 73..100 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA