pGEX4T3-HA-ERK2 K54R

53170 Arabidopsis thaliana Bacterial Expression

Plasmid Information

Purpose
Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector
Depositor
Christopher Counter
Article
Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1
Insert
ERK2 (MAPK1 Mustard Weed, Human)
Tags
GST and HA
Mutation
K54R
Available Since
June 6, 2014
Availability
Available

Sequence Information

Length
1,004 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 19..37 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
HA HA (human influenza hemagglutinin) epit… 73..100 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA