pGEX6P1-HA-MEK1 CBM

53163 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector
Depositor
Christopher Counter
Article
Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1
Insert
MEK1 (MAP2K1 Human)
Tags
GST and HA
Mutation
M187A/H188A/M230A/H239A
Available Since
June 9, 2014
Availability
Available

Sequence Information

Length
1,105 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
HRV 3C site recognition and cleavage site for human… 19..43 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
HA HA (human influenza hemagglutinin) epit… 70..97 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA